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ball mill survival of bacterial cells

In brief cell lysis and protein extraction were carried out simultaneously by phenol extraction in a ball mill. Extracted proteins were dissolved in a 2-mL aqueous solution containing 7 M urea 2 M thiourea and 0.01 g mL 1 14-dithiothreitol. Amido black assay was used to quantify protein concentration 56 57.

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